News Update on Germplasm Research: Aug – 2019

The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis

The utility of RFLP (restriction fragment length polymorphism), RAPD (random-amplified polymorphic DNA), AFLP (amplified fragment length polymorphism) and SSR (simple sequence repeat, microsatellite) markers in soybean germplasm analysis make up my mind by evaluating data content (expected heterozygosity), variety of loci at the same time analyzed per experiment (multiplex ratio) and effectiveness in assessing relationships between accessions. SSR markers have the best expected state (0.60), whereas AFLP markers have the best effective multiplex quantitative relation (19). [1]

Drought Tolerant Sorghum and Cotton Germplasm

Rosenow, D.T., Quisenberry, J.E., Wendt, C.W. and Clark, L.E., 1983. Drought tolerant sorghum and cotton germplasm. Agric. Water Manage., 7: 207–222.

Sorghum (Sorghum bichrome (L.) Moench) and cotton (Gossypium hirsutum L.) are the summer crops historically grownup within the rainfed or supplementary irrigated areas of the semi-arid southern Great Plains. each crops have the power to face up to periods of water deficit associate degreed to yield an economic come back to the farmer. [2]

Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers

Cucurbita pepo could be a extremely polymorphic species. The cultivars is classified into eight morphotypes in 2 taxonomic category, ssp. pepo and ssp. ovifera. a group of sixty nine accessions representative of the morphotypes and a few unclassified sorts was used for analysing the morphological and molecular diversity of this species. This assortment includes business cultivars and Spanish landraces, that represent the good diversification of sorts that have arisen in Europe once this species arrived from America. For the molecular variability studies, 2 PCR-based systems were utilized, AFLP and SRAP, that preferentially amplify ORFs. Principal coordinates analysis and cluster analysis mistreatment the UPGMA technique clearly separate the accessions into the 2 taxonomic category through the employment of each markers. [3]

Variability Assessment of Aromatic Rice Germplasm by Pheno-Genomic traits and Population Structure Analysis

While the pleasant scent of aromatic rice is creating it a lot of well-liked, with demand for aromatic rice expected to rise in future, styles of this have low yield potential. Genetic diversity and population structure of aromatic germplasm offer valuable info for yield improvement that has potential value and farm profit. Here, we tend to show diversity and population structure of 113 rice germplasm supported composition and genotypical traits. composition traits showed that goodish variation existed across the germplasm. [4]

Screening of Fenugreek (Trigonella foenum graecum L.) Germplasm Lines for Diosgenin Potential

Fenugreek seeds were evaluated for diosgenin content and productivity which might be helpful for pharmaceutical industries and farmers. applied mathematics significance was tested by multivariate analysis (n=5) followed by comparisons of means that by DMRT (p≤0.05). Fenugreek seeds were big within the experimental fields of Vegetable center, Pantnagar, Uttarakhand, Asian nation for cropping year 2014. 2 native varieties Pant Ragini (PR), Pusa Early Bunching (PEB) and eight accessions PM(C)1, PM 2, PM 3, PM 4, PM 5, PM 6, PM seven and PM eight were elite to guage high diosgenin content and productivity. Results indicate that fenugreek may be a sensible supply of diosgenin with high productivity, starting from zero.40 to 0.60 g/100 g and seven.78 to 14.95 Kg ha-1, severally. [5]

Reference

[1] Powell, W., Morgante, M., Andre, C., Hanafey, M., Vogel, J., Tingey, S. and Rafalski, A., 1996. The comparison of RFLP, RAPD, AFLP and SSR (microsatellite) markers for germplasm analysis. Molecular breeding, 2(3), pp.225-238. (Web Link)

[2] Rosenow, D.T., Quisenberry, J.E., Wendt, C.W. and Clark, L.E., 1983. Drought tolerant sorghum and cotton germplasm. In Developments in agricultural and managed forest ecology (Vol. 12, pp. 207-222). Elsevier. (Web Link)

[3] Ferriol, M., Pico, B. and Nuez, F., 2003. Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theoretical and Applied Genetics, 107(2), pp.271-282. (Web Link)

[4] Variability Assessment of Aromatic Rice Germplasm by Pheno-Genomic traits and Population Structure Analysis
M. Z. Islam, M. Khalequzzaman, M. K. Bashar, N. A. Ivy, M. A. K. Mian, B. R. Pittendrigh, M. M. Haque & M. P. Ali
Scientific Reportsvolume 8, Article number: 9911 (2018) (Web Link)

[5] Dwivedi, H., Singh, D. and Agrawal, S. (2017) “Screening of Fenugreek (Trigonella foenum graecum L.) Germplasm Lines for Diosgenin Potential”, Asian Research Journal of Agriculture, 4(2), pp. 1-7. doi: 10.9734/ARJA/2017/32527. (Web Link)

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